Missed the first broadcast? Join live to ask your CRISPR knock-in questions and start building better models.
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You're invited


Ask the Experts: Generating CRISPR Knock-in Cell Lines


Wednesday, October 22, 2025

8pm Amsterdam-Paris

Save Your Seat!

Join our featured panel of experts

Marie Schwinn, PhD

Senior Research Scientist

Promega

Christopher Eggers, PhD

Senior Research Scientist

Promega

This is a second live broadcast of our popular August webinar—scheduled to give you another chance to have your knock-in questions answered so you can start building your endogenous cell models with confidence.


CRISPR-based insertion of reporter tags allows researchers to study proteins in their native cellular context, providing insights into localization, interactions, and abundance without overexpression artifacts. Inserting tags such as HiBiT, NanoLuc®, or HaloTag® directly into the genome maintains natural expression while enabling sensitive detection, quantification, and imaging.


Implementing this approach requires careful choices around tags, cell background, editing tools, and validation steps. This webinar offers a short overview followed by a live Q&A, giving you direct access to expert guidance on CRISPR-based protein tagging, assay design, and biological interpretation.


Join us to connect with our experts, ask your questions live, and gain practical insights into generating CRISPR knock-in cell lines.


In this webinar, you will learn about:

  • Advantages of endogenous tagging for studying protein biology

  • Key considerations for tag knock-ins: tag choice, locus, validation

  • Real-world examples and expert insights for design, troubleshooting, and application

Register Now

Unsure what question to ask?


Successful CRISPR knock-ins depend on thoughtful choices around tag design, editing tools, and validation. Start with our Technical Application Note for key background, then join the Q&A to ask your own questions live.

Read App Note

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